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Creators/Authors contains: "Schuttler, Bernd"

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  1. ; ; ; ; ; (, MicroTAS2021)
    The use of a microwell microfluidic device allows separating single cells and tracking single cells data. The measurement of single cell fluorescent intensity trajectories in the microwell device supported a deterministic quorum sensing model identified by ensemble methods for clock phase synchronization. A strong inference framework was used to test the communication mechanism in phase synchronization of quorum sensing versus cell-to-cell contact, and the results lent support for quorum sensing. 
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    Accepted Manuscript Full Text Available
  2. ; ; ; ; ; ; (, MicroTAS 2021)
    Most eukaryotes and cyanobacterial species have a biological clock that allows adaptation to the daily light/dark cycle of the planet. A central problem in the study of the biological clock is understanding the synchro-nization of the stochastic oscillators in different cells and tissues, but this problem is largely unstudied, particularly in the context of circadian rhythms. We developed a novel microfluidic platform to make high-throughput and high-precision measurements of biological clocks on a controlled number of Neurospora crassa (N. crassa) cells. Single cell measurements in this platform enabled us to test whether clocks of individual cells are able to communicate. 
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    Accepted Manuscript Full Text Available
  3. ; ; ; ; ; (, The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2020))
    Accepted Manuscript Full Text Available
  4. ; ; ; ; ; ; (, MicroTAS2020)
    We report a microfluidic device that mimics an artificial tissue to test the theory of quorum sensing as a method for synchronization of a model fungal system, Neurospora crassa (N. crassa). High synchronicity between cells were observed by calculating the Kuramoto order parameter (K) between different fields of view.The dimensions of the microfluidic chamber allows us to also calculate an upper limit of the radius of a hypothesized quorum sensing signal by using the diffusion approximation for signal travelling within the device. 
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    Accepted Manuscript Full Text Available